| Insert |
DNA: genomic Insert lengths(kb): 14.0 Tissue: liver Gene product: DNA Segment, single copy [D15S46] Alleles: A1, A2, B1, B2, C1, C2 |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--19.5, 11.1, 9.3, 4.9; HindIII--23.1, 6.2, 3.7, 3.1, 1.0, 0.94; BamHI--15.5, 7.0, 5.6, 4.7, 4.2, 3.7; XbaI--22.0, 12.6, 9.5, 2.9, 1.2; SalI--uncut. The MspI polymorphisms have a combined heterozygosity of 26%. The MspI and HindIII polymorphisms have a combined PIC value of 0.60 and a combined heterozygosity of 62%. Enzyme(s) not detecting polymorphism: BamHI, EcoRI, TaqI, BglII (panel of 5 individuals). |
| References |
Schumm JW, et al. Identification of more than 500 RFLPs by screening random genomic clones. Am. J. Hum. Genet. 42: 143-159, 1988. PubMed: 2892396
Donis-Keller H, et al. A genetic linkage map of the huma genome. Cell 51: 319-337, 1987. PubMed: 3664638
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