Derived from Escherichia coli K-12 wild type, ATCC 25404
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
freeze-dried
Preceptrol®
no
Type Strain
no
Comments
Impaired in its ability to grow anaerobically; no nutritional requirements. High reversion frequency; reisolate mutant before use. In anaerobic jars in an atmosphere of N2 and CO2, it grows at one-fifth the rate of the wild type. Aerobic growth rate is normal
Medium
ATCC® Medium 129: Nutrient agar/stabs/broth with 0.5% NaCl
Growth Conditions
Temperature: 37°C
Name of Depositor
JH Schwartz
References
Cedar H, Schwartz JH. Production of L-asparaginase II by Escherichia coli. J. Bacteriol. 96: 2043-2048, 1968. PubMed: 4881701
Cedar H, Schwartz JH. The asparagine synthetase of Escherhic coli. I. Biosynthetic role of the enzyme, purification, and characterization of the reaction products. J. Biol. Chem. 244: 4112-4127, 1969. PubMed: 4895361
