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pPYRDH

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編號:B208056

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產品名稱	pPYRDH
商品貨號	B208056
Designations	pPYRDH
GenBank Number	M94065
Species	Homo sapiens, human
Applications	in another host, produces protein dihydroorotate dehydrogenase
Vector	Construct size (kb): 6.929999828338623 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pFL61 Intact vector size: 5.425 Type of vector: plasmid Vector end: BstXI Vector end: BstXI Cloning sites: NotI Polylinker sites: EcoRI BanII SacI Asp718 KpnI AvaI SmaI XmaI BamHI XbaI Construction: pUC19 Host range: Saccharomyces cerevisiae; Escherichia coli Features (with orientation and position when available): marker(s): ampR, URA3 replicon: pMB1, 2 micron Cross references:
Insert	DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: DHODH Gene name: dihydroorotate dehydrogenase Contains complete coding sequence?: Y Localization: 16 q22 Tissue: HeLa D98-H2 cell line Type of DNA: cDNA Insert end: Insert end: Insert size (kb): 1.5 Cross references: DNA Seq. Acc.: M94065 Insert lengths(kb): 1.5 Tissue: HeLa D98-H2 cell line Gene product: dihydroorotate dehydrogenase [DHODH] Target Gene: dihydroorotate dehydrogenase
Insert Size (kb)	1.5
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information	Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments	Restriction digests of the clone give the following sizes (kb): NotI--5.5, 1.5; BamHI--4.6, 2.3; BglI--3.6; 1.9, 1.1, 0.4; PvuII--4.8, 2.1; ClaI--4.2, 2.35, 0.5. The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--1.42; BglI--0.87, 1.25; ClaI--0.83; PstI--0.14, 0.29, 0.71; PvuII--1.06; SphI--1.3. Contains the complete coding sequence (nt 1-1191). Can complement ura1 mutants in Saccharomyces cerevisiae. Detects a unique 1.6 kb transcript in humans.
References	Minet M, et al. Cloning and sequencing of a human cDNA coding for dihydroorotate dehydrogenase by complementation of the corresponding yeast mutant. Gene 121: 393-396, 1992. PubMed: 1446837

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梅經理	17280875617	1438578920
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