The insert contains the following restriction sites (nucleotide positions according to the published sequence): PstI--130, 186; HindIII--246; SstI--403, RsaI--448, 493. Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 0.5; HindIII--3.0, 0.2; SstI--2.8, 0.4; BamHI--3.2; SmaI--3.2. This is a more complete cDNA clone than pBR322-C-A3 (ATCC 39868) or HNP-1B (ATCC 59750/1). It extends from nucleotide 22 in the published sequence. |
