Features: marker(s): kanR, strR promoter: amp, kan replicon: R300B
Applications
expression vector
shuttle vector
Comments
High copy number, double-replicon vector with a broad host range.
Media
ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C
References
Bagdasarian M, et al. Specific-purpose plasmid cloning vectors. II. Broad host range, high copy number, RSF1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas. Gene 16: 237-247, 1981. PubMed: 6282695
