The insert contains the following restriction sites, in approximate kb from the 5' EcoRI site: XhoI--0.2; PstI--0.55, 1.4; BamHI--0.85. Restriction digests of the clone give the following sizes (kb): EcoRI--3.2, 2.9; BamHI--5.2, 0.8; PstI--4.7, 0.9, 0.5. Restriction digests of the clone give the following sizes (kb): EcoRI--3.1, 2.9; BamHI--5.2, 0.8; PstI--4.7, 0.9, 0.5. Depositor verified the XhoI site. The polymorphic BglII site is within the transcribed sequence. |
