| 產(chǎn)品名稱 |
pLIN101 |
| 商品貨號(hào) |
B208708 |
| Designations |
pLIN101 |
| GenBank Number |
U15050
|
| Species |
Streptococcus agalactiae Lehmann and Neumann |
| Depositors |
DG Pritchard, B Lin |
| Applications |
produces protein hyaluronate lysase |
| Vector |
Construct size (kb): 7.931000232696533 |
| Insert |
DNA: genomic Insert lengths(kb): 3.420000076293945 Gene product: hyaluronate lysase [hylB] Target Gene: hyaluronate lysase |
| Insert Size (kb) |
3.420 |
| Media |
ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): SalI--8.0; XbaI--8.0; EcoRI--8.0; BamHI--8.0; SalI/XbaI--4.6, 3.4. The insert contains the following restriction sites (approximate kb from the 5' end): HindIII--0.35, 0.88; KpnI--1.2; EcoRI--2.19; ClaI--3.22. The insert excised with SalI/XbaI will include some vector sequence: 12 bp at the 5' end (SalI) and 36 bp at the 3' end (XbaI). |
| References |
Lin B, et al. Cloning and expression of the gene for group B streptococcal hyaluronate lyase. J. Biol. Chem. 269: 30113-30116, 1994. PubMed: 7982914
Pritchard DG, et al. Characterization of the group B streptococcal hyaluronate lyase. Arch. Biochem. Biophys. 315: 431-437, 1994. PubMed: 7986088
|
