Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
freeze-dried
Preceptrol®
no
Genotype
F- leu thi gal1 gal2 lac xyl ara hsdR deltatrpE5 lambdaDKC266(repA+)
Type Strain
no
Comments
derived from E. coli JMB9.1
Expresses the RepA protein required for replication of the P1 plasmids pVEX1211 and pVEX1212 (ATCC 77420, 77421).
Medium
ATCC® Medium 1065: LB Agar/Broth, Miller
Growth Conditions
Temperature: 37.0°C
Name of Depositor
DH Figurski
References
Ayres EK, et al. Precise deletions in large bacterial genomes by vector-mediated excision (VEX). The trfA gene of promiscuous plasmid RK2 is essential for replication in several gram-negative hosts. J. Mol. Biol. 230: 174-185, 1993. PubMed: 8450534
