| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--2.95, 0.9, 0.7; BglI--1.7, 1.3 (doublet), 0.3; PvuI--3.2, 1.1, 0.2; XbaI--3.9, 0.55; PvuII--2.4, 1.4, 0.7. The insert contains the following restriction sites (approximate kb from the T3 primer end): BglI--0.14, 0.19; PvuI--1.49; XbaI--0.02. Based on the alignment with J05224 for swine mitochondrial ac onitase (2665 nt, CDS 30-2375, approximately 80% identity over the regions sequenced), this clone appears to contain coding sequence. |
