Restriction digests of the clone give the following sizes (kb): EcoRI--4.3, 1.7; HindIII--6.1; PstI--6.0; BamHI--6.1. This clone was previously deposited as ATCC 67474. The insert contains the following restriction sites (nt position according to the published sequence): AatII--618; BamHI--688; EcoRI--973. Secretes isoamylase regulated by a promoter in the insert. The recombinant protein is recognized by anti-SMP1 isoamylase antibodies. |
