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LL288

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編號:B209348

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產品名稱	LL288
商品貨號	B209348
Designations	LL288
GenBank Number	U30610
Species	Homo sapiens, human
Depositors	LL Lanier
Vector	Construct size (kb): 4.300000190734863 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pJFE14 Intact vector size: 3.500 Type of vector: plasmid Vector end: BstXI Vector end: NotI Cloning sites: BstXI NotI SacII SfiI EcoRI Polylinker sites: BstXI NotI SacII SfiI EcoRI Construction: pcDL-SRalpha296 Host range: mammalian cells; Escherichia coli Features (with orientation and position when available): replicon: SV40 promoter for expression: SV40 early region enhancer: HTLV-1 R-U5 segment of LTR MCS: BstXI...EcoRI terminator: SV40 late region polyadenylation signal replicon: pMB1 marker(s): ampR, <- Cross references:
Insert	DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: CD94 Gene name: CD94 antigen (NK/T-cell C-type lectin receptor) ORF's seq. position: GDB probe: GDB:696221 () Contains complete coding sequence?: Y Chromosome: 12; Localization: 12 p13 Tissue: polyclonal NK cell line Type of DNA: cDNA Insert 5' end: Modification: BstXI linker Insert 3' end: Modification: NotI linker Insert size (kb): 0.8 Cross references: DNA Seq. Acc.: U30610 Nucleotides 1-834 of the insert correspond to nucleotides 1-834 of U30610. Insert lengths(kb): 0.800000011920929 Tissue: polyclonal NK cell line Gene product: CD94 antigen (NK/T-cell C-type lectin receptor) [CD94]
Insert Size (kb)	0.800
Media	ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information	Distributed: freeze-dried
Comments	Restriction digests of the clone give the following sizes (kb): BstXI--4.2; BstXI/NotI--3.4, 0.84; NotI--4.2. The insert contains the following restriction sites (approximate kb from the 5' end): KpnI--0.28; SacI--0.41.
References	Chang C, et al. Molecular characterization of human CD94: a type II membrane glycoprotein related to the C-type lectin superfamily. Eur. J. Immunol. 25: 2433-2437, 1995. PubMed: 7589107 Lanier LL, et al. Human NKR-P1A. A disulfide-linked homodimer of the C-type lectin superfamily expressed by a subset of NK and T lymphocytes. J. Immunol. 153: 2417-2428, 1994. PubMed: 8077657 Takebe Y, et al. SRalpha promoter: an efficient and versatile mammalian cDNA expression system composed of the simian virus 40 early promoter and the R-U5 segment of human T-cell leukemia virus type 1 long terminal repeat. Mol. Cell. Biol. 8: 466-472, 1988. PubMed: 2827008

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