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pGPTV-KAN

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編號:B209577

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產品名稱	pGPTV-KAN
商品貨號	B209577
Designations	pGPTV-KAN
Depositors	R Masterson, D Becker
Biosafety Level	1
Host	Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information	Size (kb): 13.5000000000000000 DESCRIPTION OF VECTOR: Intact vector size: 13.500 Type of vector: plasmid Cloning sites: EcoRI SstI SmaI XbaI SalI HindIII BamHI Polylinker sites: Construction: pBIN19, pBI101, pBIB-HYG Host range: Rhizobium radiobacter; higher plants; Escherichia coli Features (with orientation and position when available): marker(s): kanR replicon: RK2 Vector: pGPTV-KAN (plasmid) Construction: pBIN19, pBI101, pBIB-HYG Marker(s):kanR Construct size (kb): 13.5 Features: marker(s): kanR replicon: RK2
Applications	shuttle vector
Comments	Restriction digests of the clone give the following sizes (kb): EcoRI--13.6; BglII--8.2, 3.2, 2.1; HindIII--13.6. Vector contains the following restriction sites (approximate kb from the HindIII site): BamHI--1.4; EcoRI--11.4; SstI--11.7. Binary vector with plant selectable marker near the T-DNA left border and a glucuronidase (uidA) reporter gene. The marker and uidA are divergently transcribed, and can be easily removed or replaced. The order of the major features of this plasmid is: EcoRI - pAnos - SstI - uidA - SmaI - XbaI - SalI - HindIII - Pnos - BglII - nptII - BamHI - pAg7 - T-DNA - BglII - RK2 ori - kanR.
Media	ATCC^® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions	Temperature: 37.0°C
References	Becker D, et al. New plant binary vectors with selectable markers located proximal to the left T-DNA border. Plant Mol. Biol. 20: 1195-1197, 1992. PubMed: 1463855
Shipped	frozen
Shipping Information	Distributed: freeze-dried

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