| Vector Information |
Size (kb): 6.7069997787475590 DESCRIPTION OF VECTOR:
Intact vector size: 6.707
Type of vector: plasmid
Cloning sites: XbaI SpeI BamHI SmaI PstI HindIII SalI XhoI
Polylinker sites: XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI XhoI
Construction: pRS415, GAL1 promoter
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): LEU2, ->, 200-2446
terminator: CYC1, ->, 3128-3388
MCS: XhoI...XbaI, ->, 3388-3451
promoter for expression: GAL1, <-, 3452-3912
marker(s): ampR, <-, 5159-6089
centromere: CEN6/ARSH4, ->, 6193-6707 Vector: p415 GAL1 (plasmid) Promoters: Promoter for expression GAL1 Construction: pRS415, GAL1 promoter Marker(s):LEU2,ampR Construct size (kb): 6.706999778747559 Features: marker(s): LEU2
marker(s): ampR
promoter for expression: GAL1
MCS: XhoI...XbaI
terminator: CYC1
centromere: CEN6/ARSH4 |
| Comments |
Expression from the galactokinase (GAL1) promoter is tightly repressed by glucose and is strongly induced by galactose. Low copy number shuttle expression vector. One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon. |
