| 產(chǎn)品名稱 | M4A4 LM3-4 CL16 GFP |
|---|---|
| 商品貨號(hào) | B209712 |
| Organism | Homo sapiens, human |
| Cell Type | melanocyte, Melanoma |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level | 2 [Cells contain MoMuLV viral DNA sequences]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | cancer |
| Age | 31 |
| Gender | female |
| Ethnicity | Caucasian |
| Applications | Ideal for metastatic studies
|
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Derivation | M4A4 LM3-4 CL 16 GFP cell line was derived from a third generation lung metastasis after inoculation of M4A4 GFP cells in a nude mouse mammary gland.
Note: Recent studies have generated questions about the origin of the parent cell line, MDA-MB-435. Gene expression analysis of the cells produced microarrays in which MDA-MB-435 clustered with cell lines of melanoma origin instead of breast. Additional studies have since corroborated a melanocyte origin of MDA-MB-435, to which ATCC has responded by pursuing its own investigation into the identity of this cell line. The cell line to which MDA-MB-435 is reported to have been cross-contaminated with is the M14 melanoma line. |
| Clinical Data | female Caucasian 31 |
| Comments | Note: Recent studies have generated questions about the origin of the parent cell line, MDA-MB-435. Gene expression analysis of the cells produced microarrays in which MDA-MB-435 clustered with cell lines of melanoma origin instead of breast. Additional studies have since corroborated a melanocyte origin of MDA-MB-435, to which ATCC has responded by pursuing its own investigation into the identity of this cell line. The cell line to which MDA-MB-435 is reported to have been cross-contaminated with is the M14 melanoma line.
M4A4 LM3-4 CL 16 GFP (CRL-2917?) cell line was derived from a third generation lung metastasis after inoculation of M4A4 GFP cells in a nude mouse mammary gland. The M4A4 GFP (ATCC CRL-2915?) was developed by the transduction of the GFP gene into M4A4 (ATCC CRL-2914?) cell line. One of the isolated cell lines, M4A4 LM3-2 GFP (ATCC CRL-2916?) was derived from a second lung metastasis.
The parental cell line ATCC CRL-2914? (M4A4) and ATCC CRL-2918? (NM2C5) cell lines were derived from the human breast cancer cell line, MDA.MB-435. M4A4 is highly metastatic in immuno-deprived mice, while NM2C5 is weakly or virtually non-metastatic. These well characterized, tumorigenic human isogenic cell lines have dramatically opposite metastatic phenotypes and are ideal for metastatic studies |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing | Protocol: To avoid phenotypic drift it is recommended to make frozen aliquots of the cells and use each aliquot for only 10 passages. Note: If more than 5-10% non-fluorescing cells reappear at any time it is recommended to use 600 micrograms/mL G418 for 2-4 weeks to kill off the revertants. Resistance to higher concentrations of G418 depends on the number of copies of the neo resistance gene that the cell has incorporated which is not uniform for the whole population. Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:20 to 1:40 is recommended Medium Renewal: 2 to 3 times a week |
| Cryopreservation | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: 5% CO2 in air recommended Temperature: 37.0°C |
| STR Profile | Amelogenin: X CSF1PO: 11 D13S317: 12 D16S539: 13 D5S818: 11,12 D7S820: 8,10 THO1: 6,7 TPOX: 8,11 vWA: 16,18 |
| Population Doubling Time | about 24 hours |
| Name of Depositor | D Tarin |
| Year of Origin | January, 2003 |
| References | Ellison G, et al. Further evidence to support the melanocytic origin of MDA-MB-435. Mol. Pathol. 55: 294-299, 2002. PubMed: 12354931 Bao L, et al. Correlation of VLA-4 integrin expression with metastatic potential in various human tumour cell lines. Differentiation 52: 239-246, 1993. PubMed: 7683291 Urquidi V, et al. Contrasting expression of thrombospondin-1 and osteopontin correlates with absence or presence of metastatic phenotype in an isogenic model of spontaneous human breast cancer metastasis. Clin. Cancer Res. 8: 61-74, 2003. PubMed: 11801541 Goodison S, et al. Prolonged dormancy and site-specific growth potential of cancer cells spontaneously disseminated from nonmetastatic breast tumors as revealed by labeling with green fluorescent protein. Clin. Cancer Res. 9: 3808-3814, 2003. PubMed: 14506175 Montel V, et al. Expression profiling of primary tumors and matched lymphatic and lung metastases in a xenogeneic breast cancer model. Am. J. Pathol. 166: 1565-1579, 2005. PubMed: 15855655 Suzuki M, et al. Dormant cancer cells retrieved from metastasis-free organs regain tumorigenic and metastatic potency. Am. J. Pathol. 169: 673-681, 2006. PubMed: 16877365 Montel V, et al. Tumor-stromal interactions reciprocally modulate gene expression patterns during carcinogenesis and metastasis. Int. J. Cancer 119: 251-263, 2006. PubMed: 16482564 Goodison S, et al. Molecular cytogenetic analysis of a human breast metastasis model: identification of phenotype-specific chromosomal rearrangements. Cancer Genet. Cytogenet. 156: 37-48, 2005. PubMed: 15588854 Hayashi K, et al. Differential effects of retinoic acid on the growth of isogenic metastatic and non-metastatic breast cancer cell lines and their association with distinct expression of retinoic acid receptor beta isoforms 2 and 4. Int. J. Oncol. 22: 623-629, 2003. PubMed: 12579317 Tarin DTumor metastasisIn: Tarin DOxford Textbook of PathologyOxford, United KingdomOxford University Press607-663, 1992 Ross DT, et al. Systematic variation in gene expression patterns in human cancer cell lines. Nature Genetics 24: 227-235, 2000. PubMed: 10700174. Sellappan S, et al. Lineage infidelity of MDA-MB-435 cells: expression of melanocyte proteins in a breast cancer cell line. Cancer Res. 64: 3479-3485, 2004. PubMed: 15150101. Rae JM, et al. Common origins of MDA-MB-435 cells from various sources with those shown to have melanoma properties. Clin. Exp. Metastasis 21: 543-552, 2004. PubMed: 15679052. Rae JM, et al., MDA-MB-435 cells are derived from M14 Melanoma cells - a loss for breast cancer, but a boon for melanoma research. Breast Cancer Res. Treat. 104:13-19, 2007. PubMed: 17004106. |
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