Restriction digests of the clone give the following sizes (kb): EcoRI--6.6, 1.55; PstI--6.7, 1.35; PvuII--6.2, 2.05. The depositor recommends using YRp7-17camp (ATCC 37630) as the reference for CAT expression and using the CAT assay methodology of Neumann, et al. One of a series (ATCC 37624 through 37634) of Escherichia coli/Saccharomyces cerevisiae shuttle vectors for investigating control of expression in yeast. A 1520 bp NdeI fragment containing CEN6 was cloned into the NdeI site of YRp7-32phocat (ATCC 37625). |
