| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/BamHI--7.8; EcoRI--7.8; PvuII/HindIII--3.85, 3.35, 0.55; PvuII/PstI--3.45, 2.65, 1.35; ClaI--7.8; EcoRI/HindIII--3.9, 3.7; BamHI--uncut. Maps near the centromere. Polymorphisms are best resolved on 1.2% agarose gels with bromphenol blue run longer than 14 cm. RFLPs are observed under normal hybridization stringency. Washes should be done at 0.1X SSC, 0.1% SDS, 65C. EcoRI, HindIII, PvuII, BglII, PstI and BamHI neither detect the site polymorphisms nor resolve the VNTR polymorphism adequately. |
| References |
Nakamura Y, et al. Variable number of tandem repeat (VNTR) markers for human gene mapping. Science 235: 1616-1622, 1987. PubMed: 3029872
Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence (pCMI37) on chromosome 7 [D7S368]. Nucleic Acids Res. 16: 4192, 1988. PubMed: 2897676
Yusuke Nakamura, personal communication
Peter J O'Connell, personal communication
|
