| Insert |
DNA: cDNA Insert lengths(kb): 2.244999885559082 Tissue: acute T cell leukemia, Jurkat cell line Gene product: protein kinase C, alpha [PKCA] |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.3; BamHI--4.5, 0.65, 0.25; BglI--2.7, 1.3 (doublet); NcoI--4.2, 1.2; PvuII--2.4, 1.4, 0.75, 0.6. The insert contains the following restriction sites (bp from the 5' end): BamHI--585, 796; BglI--965; NcoI--26, 1178; PstI--178; PvuII--1233, 1951; SmaI--44. Contains the complete coding sequence (nt 28-2046). The insert is oriented such that transcription from T7 gives the sense strand and from T3 gives the antisense strand. |
| References |
Finkenzeller G, et al. Sequence of human protein kinase C alpha. Nucleic Acids Res. 18: 2183, 1990. PubMed: 2336401
Hubert Hug, personal communication
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