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pFL46L

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編號:B210479

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產品名稱	pFL46L
商品貨號	B210479
Designations	pFL46L
Depositors	F Lacroute
Biosafety Level	1
Vector Information	Size (kb): 7.8220000267028810 Vector: pFL46L (plasmid) Construction: pUC19 Marker(s):LEU2,ampR Construct size (kb): 7.822000026702881 Features: marker(s): ampR, LEU2 replicon: pMB1, 2 micron
Applications	YE-type (episomal) shuttle vector shuttle vector
Comments	Restriction digests of the clone give the following sizes (kb): BamHI--7.8; BglII--4.9, 2.9; ClaI--3.7, 2.3, 1.9. Contrary to published data, this vector does not permit visual detection of recombinants using the assay for alpha complementation. Shows increased stability because of the presence of STB. The loss per generation (percentage having lost all the plasmids after 20 h of non-selective growth) is 6.3. YE-type high copy number shuttle vector. The order of the major features in this plasmid is: NotI/MCS/HindIII - LEU2 - STB -2 micron ori - pMB1 ori - ampR.
Media	ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions	Temperature: 37.0°C
References	Yanisch-Perron C, et al. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103-119, 1985. PubMed: 2985470 Struhl K, et al. High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules. Proc. Natl. Acad. Sci. USA 76: 1035-1039, 1979. PubMed: 375221 Bonneaud N, et al. A family of low and high copy replicative, integrative and single-stranded S. cerevisiae/E. coli shuttle vectors. Yeast 7: 609-615, 1991. PubMed: 1767589 Chevallier MR, et al. Transcriptional and translational expression of a chimeric bacterial-yeast plasmid in yeasts. Gene 11: 11-19, 1980. PubMed: 7002730 Francois Lacroute, personal communication
Shipped	freeze-dried

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梅經理	17280875617	1438578920
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