| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--18.0, 17.0, 7.2, 4.0, 0.54; EcoRI--20.0, 11.0, 6.0, 4.4, 2.7, 1.8, 0.76; HindIII--41.0, 6.0; XbaI--20.0, 13.0, 3.2, 2.7, 2.1, 2.0, 1.5, 0.6; KpnI--19.0, 16.0, 7.6, 3.3, 1.4; SstI--20.0, 8.0, 5.8, 5.0, 3.6, 1.9. The MspI polymorphisms have a combined PIC value of 0.52 and a combined heterozygosity of 67%. Enzyme(s) not detecting polymorphism: BamHI, BglII, EcoRI, HindIII, HincII, PstI, RsaI, TaqI, MboI, HinfI, AluI, HaeIII (panel of 5 individuals). |
| References |
Schumm JW, et al. Identification of more than 500 RFLPs by screening random genomic clones. Am. J. Hum. Genet. 42: 143-159, 1988. PubMed: 2892396
Donis-Keller H, et al. A genetic linkage map of the huma genome. Cell 51: 319-337, 1987. PubMed: 3664638
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