contains easily purifiable cassette(s) for construction
Comments
Vector containing portable translation stop codons in two orientations (HindIII/stop/pots/BamHI).
The depositor has requested that any proposed commercial use of the cells be negotiated with him. The sending of these cultures does not and should not be construed to grant any license or rights to the materials to the recipient.
No distribution of the cultures or any derivatives therefrom should be made to third parties.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Pettersson RF, et al. Chemical systhesis and molecular cloning of a STOP oligonucleotide encoding an UGA translation terminator in all three reading frames. Gene 24: 15-27, 1983. PubMed: 6313480
