| 產品名稱 | U-CH1 |
|---|---|
| 商品貨號 | B211393 |
| Organism | Homo sapiens, human |
| Tissue | sacral bone |
| Product Format | frozen |
| Morphology | mesenchymal like, with variable vacuoles |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | tumor, chordoma |
| Age | 56 years |
| Gender | male |
| Ethnicity | Caucasian |
| Applications | Use as a model of chordoma which is a rare slow-growing tumor
The Chordoma Foundation may be able to offer financial assistance for the purchase of this cell line. Please contact cells@chordoma.org for more information. |
| Shipping Information | frozen |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Derivation | This cell line was established from a local recurrence of a sacrococcygeal after radiotherapy 4 years after initial surgery. |
| Clinical Data | 56 years male Caucasian |
| Genes Expressed | overexpression of transcription factor T (brachyury) |
| Comments | U-CH1 is the first human chordoma cell line. It exhibits chordoma-like characteristics and has molecular, genetic, and morphological features typical of chordoma. This cell line was established from a local recurrence of a sacrococcygeal chordoma after radiotherapy 4 years after initial surgery. Chordoma is a rare slow-growing tumor type, and U-CH1 is a relatively slow-growing cell line. U-CH1 has a heterogeneous morphology consisting of physaliferous cells with mcinous intercellular substance, which represent typical chordoma features. The cells overexpress the transcription factor T (Brachyury) that is the most specific marker for chordoma. This cell line was accessioned with the support of the Chordoma Foundation, a non-profit organization working to improve the lives of chordoma patients by accelerating research to develop effective treatments for the chordoma disease. |
| Complete Growth Medium | Iscove's Modified Dulbecco's Medium (IMDM; ATCC® No. 30-2005): RPMI-1640 Medium (ATCC® No. 30-2001) (4:1) + 10% FBS (ATCC® No. 30-2020) + additional 1% L-glutamine (ATCC® No. 30-2214) |
| Subculturing | Coating description: Dilute rat tail type I collagen (BD Biosciences, Catalog No. 354236) to 50 μg/ml. Add 7.5 ml coating buffer to flask and incubate at room temperature for one hour. Carefully aspirate remaining solution. Rinse flask 2 times to remove acid, using 1x DPBS. Coated flasks may be used immediately or stored at 2-8°C up to one week under sterile conditions. Volumes used in this subculture protocol are for a 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
|
| Cryopreservation | Freeze medium: 70% complete growth medium supplemented with an additional 20% fetal bovine serum and 10% DMSO |
| Culture Conditions | Temperature: 37oC Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile | TH01: 7 |
| Passage Number | 30 |
| Name of Depositor | Silke Bruderlein, Peter Moller |
| Deposited As | Homo sapiens |
| Year of Origin | August 17, 1998 |
| References | Scheil S, et al. Genome-wide analysis of sixteen chordomas by comparative genomic hybridization and cytogenetics of the first human chordoma cell line, U-CH1. Genes Chromosomes Cancer 32(3): 203-211, 2001. PubMed: 11579460 Bruderlein S, et al. Molecular characterization of putative chordoma cell lines. Sarcoma 2010: 630129. Epub 2010 Dec 30. PubMed: 21253487 Presneau N, et al. Role of the transcription factor T (brechyury) in the pathogenesis of sporadic chordoma: a genetic and functional-based study. J. Pathol. 2239(3): 327-335, 2011. PubMed: 21171078 Liu X, et al. Establishment and Characterization of a Novel Chordoma Cell Line: CH22. J. Orthip Res. 30 (10): 1666-1673, 2010. PubMed: 22504929 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
