Restriction digests of the clone give the following sizes (kb): PstI--7.0; PvuI--7.0; SmaI--7.0; BamHI--4.7, 2.4. Cloning into the HincII, PstI, PvuI, or ScaI sites inactivates ampR. Cloning into the SmaI site inactivates cmlR. Precautions have not been taken to maintain the F' in the JM109 host. The F' is not required to use this plasmid. Shuttle vector. The order of the major features in this plasmid is: pMB1 ori - ampR - pRSF0885 rep - cmlR. |
