Host should be mk+ because some packaging mixes destroy charomids by EcoK.
Useful for cloning 18-32 kb fragments.
Comments
Dimer vector can be packaged, so cosmid-type 'arm' preparation or phosphatase treatment is necessary. Host should be mk+ because some packaging mixes destroy charomids by EcoK.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Useful for cloning 18-32 kb fragments.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 30.0°C
References
Saito I, Stark GR. Charomids: Cosmid vectors for efficient cloning and mapping of large or small restriction fragments. Proc. Natl. Acad. Sci. USA 83: 8664-8668, 1986. PubMed: 3022301
