| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--7.0, 3.5 (doublet), 2.85 (doublet), 2.55 (doublet), 2.4, 1.55 (triplet), 1.2 (doublet) and smaller bands. Reported to contain EcoRI/HindIII fragments of the following sizes (kb): 6.724, 3.505, 3.505, 2.922, 2.899, 2.772 (vector), 2.547, 2.547, 2.387, 1.630, 1.569, 1.569, 1.238, 1.222, 0.827, 0.777, 0.697, 0.642, 0.494, 0.430, 0.429 (vector). IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Corresponds to nt 270879-311407 of the sequence of chromosome VIII. |
| References |
Gimeno CJ, Fink GR. Induction of pseudohyphal growth by over expression of PHD1, a Saccharomyces cerevisiae gene related to transcriptional regulators of fungal development. Mol. Cell. Biol. 14: 2100-2112, 1994. PubMed: 8114741
Linda Riles, personal communication
|
