| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--2.8, 1.0, 0.4; PstI--3.3, 0.7, 0.2; XhoI/HindIII--2.8, 1.5; BglI--2.8, 1.3, 0.3; HaeII--2.0, 1.4, 0.7, 0.4. The insert contains the following restriction sites [approximate kb from the 5' end (published nt position)]: PstI--0.3 (+413), 1.0 (+1119), 1.2 (+1318); EcoRI--1.0 (+1109). Contains the last 1.435 kb of the cDNA, including an ORF of 1.194 kb. Lacks the 5' untranslated region and sequences encoding the first 36 aa. Detects a mRNA of 1.7 kb in livers of normal and clofibrate-treated rats. |
