| Applications |
in another host, produces protein glucosidase, beta; acid Glucosidase, beta, acid, glucocerebrosidase |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--11.5; HindIII--4.0, 3.5, 1.9, 1.1, 1.0; PstI--11.0, 0.3; PvuII--3.5, 3.1, 2.4, 1.4, 0.7, 0.3. The glucocerebrosidase insert contains the following restriction sites (approximate kb from the 5' end): HindIII--0.83; PstI--0.27, 0.56; SalI--1.25; BamHI--1.48. Contains the complete coding sequence for glucocerebrosidase (nt 6-1556), plus 290 nt at the 3' end. Corresponds approximately to nt 490-2336 of the sequence record. pVL941 is a transfer vector encoding polyhedrin and containing its complete 5' non-coding regulator sequence. |
