Restriction digests of the clone give the following sizes (kb): EcoRI--2.7; PvuI--1.75, 0.9; HindIII--2.7; NotI--2.7. Available for research purposes only. General purpose cloning vector containing a unique NotI site. Constructed for insertion of mobilization cassettes pMOB1-4 (ATCC 77280-77283) to produce a vector useful for allele replacement in Pseudomonas aeruginosa. Also permits visual detection of recombinants by lacZalpha complementation. The alpha peptide is 107 aa. |
