| Insert |
DNA: genomic Insert lengths(kb): 40.0 Tissue: DNA from 762-8A hybrid cell line Gene product: DNA Segment, single copy [D10S34] Alleles: A1, A2, B1, B2 |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--19.0, 10.0, 8.2, 7.6; EcoRI--8.2 (doublet), 7.6, 4.4, 4.0, 3.7, 3.2, 2.0; BglI--7.0, 6.6 (doublet), 6.0, 5.4, 4.6, 3.7, 2.3, 2.0, 1.4; SalI--25.0, 19.0; NotI--uncut; HindIII--13.0, 6.6, 6.0, 3.7, 3.4 (doublet), 2.3, 1.8, 1.3. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. |
