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pRML2

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編號:B212232

品牌:Mingzhoubio

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產品名稱	pRML2
商品貨號	B212232
Designations	pRML2
Depositors	F Spencer
Biosafety Level	1
Vector Information	Size (kb): 5.0999999046325680 Vector: pRML2 (plasmid) Promoters: Promoter for in vitro transcription T7 Construction: pBluescript + URA3 + ARSH4 Marker(s): ampR Features: marker(s): URA3 marker(s): ampR *promoter for in vitro* transcription: T7 replicon: ARSH4 replicon: ori restriction site: SalI coding sequence:** TEL
Applications	cloning vector
Comments	Restriction digests of the clone give the following sizes (kb): BamHI--5.1; EcoRI--5.1; NotI--5.1; XbaI--5.1; EcoRI/XbaI--4.5, 0.5. YAC cloning vector. The plasmid was from pBluescript, and includes a URA3 selectable marker, yeast origin of replication ARSH4, a distal polylinker to support plasmid rescue of insert ends, T7 promoter, and a cloning polylinker for library production.
Media	ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions	Temperature: 37°C
References	Spencer F, et al. Targeted recombination-based cloning and manipulation of large DNA segments in yeast. Methods 5: 161-175, 1993. Smith DR, et al. Copy number amplification of yeast artifical chromosomes. Methods Enzymol. 216: 603-614, 1992. PubMed: 1336106 Zhong TP, et al. Zebrafish genomic library in yeast artifical chromosomes. Genomics 48: 136-138, 1998. PubMed: AMBIGUOUS 95 Haldi ML, et al. A comprehensive large-insert yeast artificial chromosome library for physical mapping of the mouse genome. Mamm. Genome 7: 767-769, 1996. PubMed: 8854865
Shipped	freeze-dried
Shipping Information	in E. coli containing the plasmid

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梅經理	17280875617	1438578920
胡經理	13345964880	2438244627
周經理	17757487661	1296385441
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沈經理	19548299266	2662369050
李經理	13626845108	972239479

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