Store Trypsin-EDTA solution frozen, between -5°C and -40°C. Avoid repeated freeze-thaws by dispensing and storing in aliquots.
Applications
0.25% Trypsin/0.53 mM EDTA in Hanks Balanced Salt Solution without calcium or magnesium. For dissociation of cell monolayers. Trypsin-EDTA solution is suitable for most but not for all adherent cell lines. For cell line-specific information, please go to the appropriate product page on the web, refer to the product sheet supplied with the cell line, or contact ATCC Technical Service.
Comments
NOTE: Each cell line responds to ATCC® Trypsin-EDTA solution in a unique manner. For optimum results, observe the cells during the dissociation process to avoid over-trypsinization. For more information, please refer to the General Protocol for Using ATCC® Trypsin-EDTA Solution. For cell line-specific information, please refer to the product sheet supplied with the cell line, or contact ATCC® Technical Service. ATCC® Trypsin-EDTA solution is suitable for most but not for all adherent cell lines. This product contains phenol red.
Biosafety Level
N/A
Quality Control Specifications
Lot specific results are available in the Certificate of Analysis, which is available at www.atcc.org or by contacting ATCC technical service.
