| 產品名稱 |
E1.4 |
| 商品貨號 |
B212360 |
| Designations |
E1.4 |
| Species |
Mus musculus, mouse |
| Depositors |
V Ramamurthy |
| Vector |
Construct size (kb): 4.400000095367432 |
| Insert |
DNA: genomic Insert lengths(kb): 1.399999976158142 Tissue: B-1/200 fibroblast cell line Gene product: adenosine deaminase [Ada] |
| Insert Size (kb) |
1.400 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.2, 1.4; HindIII--4.5; BamHI--4.5; PstI--4.5; XbaI--4.5. Contains exon 1 and flanking sequences. Within this clone are sequences which appear to signal termination of transcription, regulating gene expression. The insert contains a SmaI site at nt 79, where nt 1 is the A of the initiation codon. The insert extends from nt -241 to nt +1155. |
| Classification |
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References |
Ramamurthy V, et al. Sequence requirements for transcriptional arrest in exon 1 of the murine adenosine deaminase gene. Mol. Cell. Biol. 10: 1484-1491, 1990. PubMed: 1690842
Maa MC, et al. Identification of transcription stop sites at the 5' and 3' ends of the murine adenosine deaminase gene. J. Biol. Chem. 265: 12513-12519, 1990. PubMed: 2373702
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