| 產品名稱 | Leishmania major (Yakimoff and Schokhor) Bray et al. |
|---|---|
| 商品貨號 | B212383 |
| Deposited As | Leishmania major NIH SD (MHOM/SN/74/SD) |
| Strain Designations | MHOM/SN/74/SD |
| Application | Wild type strain used in the targeted deletion of leishmanolysin (GP63) Ref  Joshi, PB, et al. Targeted gene deletion in Leishmania major identifies leishmanolysin (GP63) as a virulence factor. Mol. Biochem. Parasitol. 120: 33-40, 2002. PubMed: 11849703 |
| Biosafety Level | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation | Cutaneous leishmaniasis, Senegal, 1973 |
| Product Format | frozen |
| Storage Conditions | Frozen: -70°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Protocols Section |
| Type Strain | no |
| Comments | The strain has also been referred in the scientific literature as NIH S strain (MHOM/SN/74/Seidman) RefNeva FA, et al. Cutaneous leishmaniasis--a case with persistent organisms after treatment in presence of normal immune response. Am. J. Trop. Med. Hyg. 28: 467-71, 1979. PubMed: 222157
RefMcDowell, MA, et al. Leishmania priming of human dendritic cells for CD40 ligand-induced interleukin-12p70 secretion is strain and species dependent. Infect. Immun. 70: 3994-4001, 2002. PubMed: 12117904 |
| Medium | ATCC® Medium 2736: M199, Modified Medium ATCC® Medium 1011: Diphasic blood agar medium ATCC® Medium 807: Brain heart infusion blood agar |
| Growth Conditions | Temperature: 20°C to 25°C |
| Cryopreservation | Harvest and Preservation
|
| Name of Depositor | R McMaster |
| Chain of Custody | ATCC <-- R McMaster <-- NIH |
| References | Neva FA, et al. Cutaneous leishmaniasis--a case with persistent organisms after treatment in presence of normal immune response. Am. J. Trop. Med. Hyg. 28: 467-71, 1979. PubMed: 222157 McDowell, MA, et al. Leishmania priming of human dendritic cells for CD40 ligand-induced interleukin-12p70 secretion is strain and species dependent. Infect. Immun. 70: 3994-4001, 2002. PubMed: 12117904 Joshi, PB, et al. Targeted gene deletion in Leishmania major identifies leishmanolysin (GP63) as a virulence factor. Mol. Biochem. Parasitol. 120: 33-40, 2002. PubMed: 11849703 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
