The recommended titer for Western blotting is 1:5000.
Plant research
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
The nSM gene of the L3 isolate of TSWV was expressed using vector pET28a and bacterium E. coli HMS174 (DE3) and protein purified using His-column.
Rabbits were inoculated with the purified recombinant protein.
The crude serum was cross absorbed with lysed bacteria, and the IgG purified using a Protein A column.
Product Format
frozen
Comments
The nSM gene of the L3 isolate of TSWV was expressed using vector pET28a and bacterium E. coli HMS174 (DE3) and protein purified using His-column.
Rabbits were inoculated with the purified recombinant protein.
The crude serum was cross absorbed with lysed bacteria, and the IgG purified using a Protein A column.
The antiserum is frozen with 50% glycerol and 0.05% sodium azide.
The recommended titer for Western blotting is 1:5000.
Name of Depositor
UB Gunasinghe
References
Soellick TR, et al. The movement protein NSm of tomato spotted wilt tospovirus (TSWV): RNA binding, interaction with the TSWV N protein, and identification of interacting plant proteins. Proc. Natl. Acad. Sci. USA 97: 2373-2378, 2000. PubMed: 10688879
