Replication has not been observed in amphibian or mammalian cell lines.
Biosafety Level
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
Whole victim homogenates of trout with infectious pancreatic necrosis, Buhl, Idaho
Product Format
frozen
Storage Conditions
-70°C or colder
Comments
Replication has not been observed in amphibian or mammalian cell lines. Brook trout 4 weeks of age die 1-4 weeks after feeding on the virus. Stable at -20°C ( 7 years) or at 4°C ( in 50% glycerol). This is a serious salmonid fish pathogen.
Effect on Host
Cytopathic effects cytoplasmic contraction and cell lysis
Recommended Host
rainbow trout gonad (RTG-2) cells (ATCC CCL-55)
RTG-2 cells (ATCC CCL-55); other fish cell lines; Salmonoid fishes
Growth Conditions
Temperature: 21°C
Incubation: 2-3 days
Name of Depositor
KE Wolf, MC Quimby, ATCC
Source
Whole victim homogenates of trout with infectious pancreatic necrosis, Buhl, Idaho
References
Wolf K, Quimby MC, Bradford AD. Egg-associated transmission of IPN virus of trouts. Virology 21: 317-321, 1963.
