| 產(chǎn)品名稱(chēng) |
pJJ244 |
| 商品貨號(hào) |
B212756 |
| Designations |
pJJ244 |
| Species |
Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors |
L Prakash |
| Vector |
Construct size (kb): 3.900000095367432 |
| Insert |
DNA: genomic Insert lengths(kb): 1.166000008583069 Gene product: uridine monophosphate synthetase( UMP synthase, orotate phosphoribosyltransferase, orotidine 5'-phosphate decarboxylase, orotate phosphoribosyltransferase 1) [URA3] |
| Insert Size (kb) |
1.166 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): HindIII--2.7, 1.2; PvuII--2.35, 1.5; EcoRI--3.9. A plasmid providing the gene for a yeast selectable marker in the MCS of pUC18, allowing more flexibility in designing constructions. URA3 is in an orientation opposite to lacZ'. The insert was subcloned from YIp5. |
| Classification |
Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References |
Jones JS, Prakash L. Yeast Saccharomyces cerevisiae selectable markers in pUC18 polylinkers. Yeast 6: 363-366, 1990. PubMed: 2220072
Riles L, et al. Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6-kilobase pairs. Genetics 134: 81-150, 1993. PubMed: 8514151
|
