The cloning sites in this vector are 5' to a promoterless beta-galactosidase gene. The insert must provide the promoter, the ribosome binding site, and/or the ATG translation initiation codon for activity to be expressed.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Shultz J, et al. A previously unidentified gene in the spc operon of Escherichia coli K12 specifies a component of the protein export machinery. Cell 31: 227-235, 1982. PubMed: 6297749
