Restriction digests of the clone give the following sizes (kb): PvuI--1.8, 1.0; EcoRI--2.8; HindIII--2.8; SspI--2.8; ClaI--2.8. The plasmid contains the following restriction sites (bp from 0): NotI--0; Nsi--75; PvuI--119, 1907; EcoRI--237; HindIII--288; SapI--524; DrdI--749, 2618; BsaI--1607; SspI--2342. Cloning vector with a second multiple cloning site inserted at the KasI site of pUC19 not interrupting lacZ'. The order of the major features in this plasmid is: NotI/MCSII/NsiI - 3' lacZ' - EcoRI/MCSI/HindIII - 5' lacZ' - SapI - pMB1 ori - ampR. |
