| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 2.5, 0.6; PstI--3.1, 2.0, 0.8; SalI--5.6, 0.3. The insert contains the following restriction sites (kb from the EcoRI site 10 bp 5' to the BamHI/Sau3AI junction): SalI--0.26, 0.52, 0.85; PstI--1.0, 1.8. Expression is regulated by the lac promoter, and is low in the absence of inducer. |
| References |
Somerville CR, Somerville SC. Cloning and expression of the Rhodospirillum rubrum ribulosebisphosphate carboxylase gene in E. coli. Mol. Gen. Genet. 193: 214-219, 1984.
Nargang F, et al. Nucleotide sequence of the ribulosebisphosphate carboxylase gene from Rhodospirillum rubrum. Mol. Gen. Genet. 193: 220-224, 1984.
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