Restriction digests of the clone give the following sizes (kb): EcoRI/XbaI--3.0, 1.l; PstI--3.8, 0.3; HindIII--4.1; BamHI--3.5, 0.6; SmaI--4.1. The insert contains the following restriction sites (nt position according to the published sequence): PstI--181, 449; BamHI--498, 1083; SmaI--576. Corresponds to nt 31 - 1144 of the published sequence, and contains the complete coding region (nt 47 - 1054). Transcription from the T7 promoter gives the sense strand. |
