ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.95 doublet; HindIII--5.8; PstI--5.8; BamHI--5.0, 0.8; SalI--5.8.
This clone includes exons 2 - 13 plus extraneous sequences of unknown origin at the 5' end. It hybridizes to genomic EcoRI fragments of 8.5, 4.6, 4.7, 0.5, 5.2, 9.3, 10.3, 4.3, 3.5, 2.4, 0.5, and 1.8 kb.
References
Riordan JR, et al. Identification of the cystic fibrosis gene: cloning and characterization of complementary DNA. Science 245: 1066-1073, 1989. PubMed: 2475911
