Restriction digests of the clone give the following sizes (kb): EcoRI--7.2, 2.1; XhoI--7.9, 1.1, 0.3; NotI/SalI--5.9, 3.4. The insert contains the following restriction sites (approximate kb from the 5' end): EcoRI--2.08; XhoI--2.28; SmaI--2.91. Expression is regulated by the CMV promoter. The order of the major features in this plasmid is: SalI - MCT1 - EcoRI - neo - ampR - CMV promoter. |
