| Comments |
Depositor indicates the following restriction digests (kb): EcoRI--4.3, 1.39; EcoRI+BglII--4.3, 1.18, 0.207; AvaI--2.5, 3.25; AccI--3.12, 1.6, 1.07; AvaII--1.75 (doublet), 1.07, several<0.3; EcoRI+AvaII--1.75, 0.96, 0.8, 0.63, 0.4, several smaller. Restriction digests of the clone give the following sizes (kb): EcoRI--4.5, 1.39; HindIII--6.2. The insert is a subclone of lambdaHPT23 (ATCC 37598) and contains the promoter and exon 1 of the gene (105 bp of 5' untranslated sequence plus sequence coding for the first 9 amino acids). |
| References |
Melton DW, et al. Structure, expression, and mutation of the hypoxanthine phosphoribosyltransferase gene. Proc. Natl. Acad. Sci. USA 81: 2147-2151, 1984. PubMed: 6326107
C. Thomas Caskey, personal communication
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