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pKi-67/1

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編號:B213921

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產品名稱	pKi-67/1
商品貨號	B213921
Designations	pKi-67/1
GenBank Number	X65550
Species	Homo sapiens, human
Depositors	J Gerdes
Vector	Construct size (kb): 6.800000190734863 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBR325 Intact vector size: 5.996 Type of vector: plasmid Vector end: EcoRI Vector end: EcoRI Cloning sites: AatII AsuII AvaI ClaI HgiEII HindIII Tth111I BamHI EcoRI EcoRV (MstI-Cm?) NcoI PstI PvuI SalI SphI XmaIII XmnI Polylinker sites: Host range: Escherichia coli Features (with orientation and position when available): marker(s): ampR, cmLR, tetR replicon: pMB1 Cross references:
Insert	DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: MKI67 Gene name: antigen identified by monoclonal antibody Ki-67 Contains complete coding sequence?: N Chromosome: 10; Localization: 10 q25-qter Tissue: IM-9 cell line Type of DNA: cDNA Insert end: Modification: EcoRI linkers Insert end: Modification: EcoRI linkers Insert size (kb): 1.092 Cross references: DNA Seq. Acc.: X65550 Insert lengths(kb): 1.092000007629395 Tissue: IM-9 cell line Gene product: antigen identified by monoclonal antibody Ki-67( Proliferation-related Ki-67 antigen, Ki-67 antigen) [MKI67]
Insert Size (kb)	1.092
Media	ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information	Distributed: freeze-dried
Comments	Restriction digests of the clone give the following sizes (kb): EcoRI--6.0, 1.1; HindIII--7.0; PstI--5.0, 2.1; SacI--7.1; XbaI--7.1. The insert contains the following restriction sites (approximate kb from the 5' end): PstI--1.00; SacI--0.21, 0.57, 0.73, 0.94; XbaI--0.18. It includes three of sixteen Ki-67 repeats. Detects splicing isoforms of 8.7 and 9.8 kb.
References	Fonatsch C, et al. Assignment of the human Ki-67 gene (MKI67) to 10q25-qter. Genomics 11: 476-477, 1991. PubMed: 1769665

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