| 產品名稱 | UPCI:SCC152 |
|---|---|
| 商品貨號 | B213976 |
| Organism | Homo sapiens, human |
| Tissue | Derived from: hypopharynx |
| Cell Type | squamous cell |
| Product Format | frozen 1 mL |
| Morphology | epithelial-like |
| Culture Properties | adherent |
| Biosafety Level | 2 [Cells contain human papillomavirus (HPV) DNA sequences]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | squamous cell carcinoma; recurrent, grade 2 |
| Age | 47 |
| Gender | male |
| Ethnicity | Caucasian |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Derivation | The surgically excised tumor was minced and distributed into flasks. When growth of fibroblasts obstructed the growth of the squamous cells, the fibroblasts were removed by trypsinization. The remaining squamous cells were grown and passaged. |
| Clinical Data | 47 years
Caucasian
male
The donor smoked tobacco, drank alcoholic beverages, and died of his disease. |
| Oncogene | amplification of chromosomal band 11q13, not expressed mutations of TP53 codon, not expressed |
| Comments | UPCI:SCC152 is from a squamous cell carcinoma of the hypopharynx. It was a recurrent tumor. The patient’s original tumor site was the base of the tongue. The UPCI:SCC152 cells are positive for Human Papilloma Virus (HPV). These cells have no TP53 mutations as assayed by sequencing the 5-8 exons of TP53. UPCI:SCC152 showed no amplification of chromosomal band 11q13 using FISH. The base of the tongue squamous cell carcinoma is ATCC CRL-3239, UPCI:090. These 2 HNSCC (head and neck squamous cell carcinoma) cell lines are a resource for studying the initiation, cancerization, prognosis, intervention and treatment of oral cancers. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium (ATCC® 30-2003™). To make the complete growth medium, add the following components to the base medium:
|
| Seeding Density | 3 x 104 viable cells/cm2 |
| Subculturing | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:2 to 1:4 is recommended.
Medium renewal: 2 to 3 times a week |
| Maintenance | The flask was seeded with cells (see specific batch information) grown and completely filled with medium at ATCC to prevent loss of cells during shipping.
|
| Volume | 1 mL |
| STR Profile | TH01: 7, 9.3 D5S818: 11, 12 D13S317: 11 D7S820: 9, 10 D16S539: 12,13 CSF1PO: 11,12 Amelogenin: X,Y vWA: 17 TPOX: 8 |
| Name of Depositor | Susanne M. Gollin, University of Pittsburgh |
| Year of Origin | February 1996 |
| References | White JS, et al. The influence of clinical and demographic risk factors in the establishment of head and neck squamous cell carcinoma cell lines. Oral Oncol. 43:701-712, 2007. PubMed: 17112776 Martin CL, et al. Chromosomal imbalances in oral squamous cell carcinoma: examination of 31 cell lines and review of the literature. Oral Oncol. 44:369-382, 2008. PubMed: 17681875 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
