| 產品名稱 |
pCM190 |
| 商品貨號 |
B214030 |
| Designations |
pCM190 |
| Depositors |
E Herrero |
| Biosafety Level |
1 |
| Vector Information |
Size (kb): 8.0120000839233400 Vector: pCM190 (plasmid) Promoters: Promoter CMV Marker(s):URA3,ampR Construct size (kb): 8.01200008392334 Features: marker(s): URA3
marker(s): ampR
operator: tetO - seven repeats
other: tTA transcriptional activator
promoter: CMV
promoter: CYC1
replicon: 2 micron
replicon: pMB1
MCS: BamHI...PstI
terminator: ADH1
terminator: CYC1 |
| Applications |
YE-type (episomal) shuttle vector expression vector |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--8.0; PstI--8.0; BamHI/XhoI--7.2, 0.7. Yeast episomal expression vector driven by the tetO-CYC1 promoter. One of 7 tetracycline-regulated expression vectors (ATCC 87656 - 87662) differing in the level of expression, mode of replication and selectable marker. |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Growth Conditions |
Temperature: 37.0°C |
| References |
Gari E, et al. A set of vectors with a tetracycline-regulatable promoter system for modulated gene expression in Saccharomyces cerevisiae. Yeast 13: 837-848, 1997. PubMed: 9234672
|
| Related Products |
component of:ATCC 87663 |
| Shipped |
frozen |
| Shipping Information |
?Frozen glycerol stock of Escherichia coli DH5alpha containing the plasmid. |
