| 產品名稱 |
pPR969 [NEB687] |
| 商品貨號 |
B214164 |
| Designations |
pPR969 [NEB687] |
| GenBank Number |
M74198
|
| Species |
Thermococcus litoralis Neuner et al. |
| Depositors |
New England Biolabs, Inc., I Schildkraut, New England Biolabs, Inc. |
| Vector |
Construct size (kb): 10.1 |
| Insert |
DNA: genomic Insert lengths(kb): 3.824 Gene product: DNA polymerase (Vent) [pol] |
| Insert Size (kb) |
3.900 |
| Media |
ATCC® Medium 1315: LB Medium (ATCC medium 1065) with 100 mg/L ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Shipped: ?Freeze dried E. coli containing the plasmid |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--11.5; EcoRI--9.4, 2.0; HindIII--10.75, 0.68. Constructed by modifying the sequence TTTATG to CATATG to create a NdeI site at the 5' end (nt 288-293), and by removing the intervening sequence spanning nucleotides 1776 and 3398 of the sequence record. |
| References |
Comb DG, et al. Purified thermostable DNA polymerase obtainable from Thermococcus litoralis. US Patent 5,322,785 dated Jun 21 1994
Comb DG, et al. Recombinant thermostable DNA polymerase from archaebacteria. US Patent 5,352,778 dated Oct 4 1994
Perler FB, et al. Intervening sequences in an Archaea DNA polymerase gene. Proc. Natl. Acad. Sci. USA 89: 5577-5581, 1992. PubMed: 1608969
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
