Restriction digests of the clone give the following sizes (kb): EcoRI--5.5; HindIII--5.5; BamHI--5.5; PstI--4.3, 1.2; AvaI--5.5. DNA containing this sequence is underrepresented in most genomic libraries because most restriction enzymes (except HinfI and TaqI) generate fragments with pHuR195 elements larger than 20 kb. This repeat is a variant of the satellite 2 family with a core sequence of CATCAT followed by 4 divergent GGAAT elements with a spacing of 0 - 49 nt. |
