| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--uncut; EcoRI--5.2; HindIII--5.2; SalI--5.2; HindIII/XhoI--5.2; XhoI--uncut; HindIII/SalI--3.6, 1.7; HindIII/EcoRI--3.6, 1.5. The BamHI insert site was lost during construction. The HindIII/XhoI double digest releases the insert plus about 0.5 kb of the vector. Enzyme(s) not detecting polymorphism: MboI, MspI, EcoRI, HindIII, PvuII, BglII, BclI, HincI, PstI, SstI, XmaI, AccI, StuI, RsaI, EcoRV, ApaI, NcoI, TthI, XmnI, BstXI. |
| References |
Barker D, et al. Restriction sites containing CpG show a higher frequency of polymorphism in human DNA. Cell 36: 131-138, 1984. PubMed: 6198090
Naylor SL, et al. DNA polymorphic loci mapped to human chromosome 3, 5, 9, 11, 17, 18, and 22. Proc. Natl. Acad. Sci. USA 81: 2447-2451, 1984. PubMed: 6585809
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