Restriction digests of the clone give the following sizes (kb): HindIII--5.2; EcoRI--5.2, HindIII/EcoRI--2.7 doublet; BamHI--2.7 doublet; SalI--5.2. Insert contains MspI (1), PstI (2), HindII (2) and HindIII (1) sites. The clone includes part of intron 8, exon 9, and 3' flanking sequences, with 2 putatitve poly(A) signals. Shows 90% homology with coding region of hamster vimentin. Positions of non-coding regions conserved, but less than 45% homology between the human and hamster intron sequences. This probe is derived from the coding region of a larger genomic probe lambdaV8. |
