A set of plasmids to be used as controls for detecting mutations or validating methods of mutation scanning.
These plasmids are designed so that the same PCR primer pair can be used on each plasmid.
Comments
Primers have been tested that yield DNA fragments sizes ranging between 203 and 1455 bp.
A set of plasmids to be used as controls for detecting mutations or validating methods of mutation scanning. The mutations are either point variants (A, T, C or G at nt 272) or insertions of one to five nucleotides (starting at nt 274).
The plasmids were derived from pUC19 (GenBank M77789), with each plasmid containing a 10 to 15 base pair replacement between the BamHI and EcoRI sites. These plasmids are designed so that the same PCR primer pair can be used on each plasmid.
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
